Purpose: The purpose of this study was to explore the participation motivation, barrier factors, and enduring involvement in bicyclists. Methods: Based on the qualitative methods, five experienced bicyclists were interviewed by judgment sampling and using semi-structured interview, documents analysis, and two experienced bicyclists were observed their actual participation behaviors for six weeks by participation observation. All of the data were rearranged, induced, compared, and analyzed by open coding, axial coding, and selective coding. Results: The results of this study indicated that the participation motivations of bicyclists included social involvement, physical fitness and emotional compensation. The barrier factors included structural barriers, interpersonal barriers and social barriers. The enduring involvement included self adjustment, self fulfillment and self challenge. Conclusion: The main factors of riding for bicyclists were to promote their fitness, social relationships, and reduce stress. They were usually constrained by family, work, social interaction, climates, and equipments. By reason thinking, positive attitude, overcome barriers, enthusiasm, and self expectancy could enhance the bicyclists’ enduring involvement.