A fairy tale is composed of a fictional plot, unique characters and events. When converting a story into an image, designers give life to the image, which can draw consumer attention and induce development of related issues and products after publicized. Through a survey of the symbols of "Cinderella", this paper aims to investigate the image expressing in posters of the Disney Animation "Cinderella" and explore the differences between consumers and Disney animation designers in perceptions of symbols. Data were collected through a questionnaire and classified through content analysis. Readers’ preferences for the story and perceptions of symbols were analyzed by their background variables to find out the representative symbols of characters, events, and objects (such as the glass slipper or animals). Results include: (1) Through a survey on perceptions of 145 respondents, 769 symbols were collected and classified into 21 categories of symbols; (2) The image expressing in 45 samples in Cinderella was analyzed based on the 21 categories of symbols. The cross-analysis result indicates that "glass slipper, pumpkin coach, small animals, Cinderella, and the prince" are representative symbols of Cinderella. The above results can be a reference for readers, graphic designers, and related industries.