Purposes: this study was to examine the levels of serious leisure, recreation specialization and perceived flow experiences during activity participation, and further identify the relationships between these three variables. Methods: A survey questionnaire was employed and data were collected on members of bicycle riders associations from north, central, south and east regions of Taiwan. Six hundred and twenty-seven questionnaires were distributed and 605 were retained. Data collected were analyzed using descriptive statistics, factor analysis, and regression analysis. Conclusion: The major findings were summarized as follows: (1) The characteristics of serious leisure possessed by bicycle riders significantly predicted recreation specialization, with durable benefits, need to persevere /personal effort the most powerful predictor; (2) The level of recreation specialization possessed by bicycle riders significantly predicted their flow experiences, with skill and knowledge the most powerful predictor; (3) The characteristics of serious leisure possessed by bicycle riders significantly predicted their flow experiences, with persevere and personal effort the most powerful predictor. The findings suggested that bicycle riders can obtain flow experiences by enhancing their levels of serious leisure and recreation specialization.