Recent changes in travel patterns have made working as a tour guide more challenging. When tour guides cannot cope with their occupational stress, their service quality and intentions to remain working for their organization are negatively affected. Therefore, this study examined the current status and differences in the quality of working life (QWL) and turnover intentions of tour guides. Moreover, the correlation between the QWL and turnover intentions was analyzed. Questionnaire survey of tour guides as the study population was conducted, and a total of 179 valid questionnaires were obtained. The data analysis revealed that the mean scores for QWL and turnover intentions were 3.45 and 3.01, respectively. The differences in the tour guides’ personal backgrounds had a significant effect only on their QWL, and their QWL significantly influenced their turnover intentions. The combined predictive effect of these factors on turnover intentions was identified, the coefficient of multiple correlations was 0.48, and the joint variance explained was 23%. Finally, the following three practical suggestions are proposed: (a) a comprehensive, fair, and reasonable compensation and reward system must be established; (b) approaches for systematic career advancement and communication channel must be formulated; and (c) an employee assistance program should be established to enable employees to balance their work and life.