The purpose of this study attempted to understand the experience of the masculinity practice of the four male nurses in the psychiatric acute wards in a hospital, regarding their gender in the "feminine" work place. Ethnography was employed as the research method.The findings of this study indicate that the masculinities of the four male nurses exhibited diversity: a flexible and "handy/useful" macho man; androgynous expanded care work; overwhelming toughness on conflicts and exhibition of physical strength for masculinity; and controlling "power" over female colleagues exhibited manliness. In particular, tough and powerful masculinities include collectiveness and normativity, impacting each individual's need to identify the male traits of "being strong and having strength" in order to overcome his sense of fear of being different within the feminine work place.We conclude that males entering the traditional female workplace may face sex discrimination and biases and encounter some unequal treatments and difficulties according to the masculinity practice experiences of these four male nurses. We expect a decline in the dominant ideology of sex-dualism and recognize the male nurses' professional labor in order to establish a gender friendly culture and environment of hospital organizations leading to greater gender equality.