Students of the Department of Physical Education are presented masculine flavor and have a masculine image in the traditional stereotypes. However, impacted by society and environment, the men and women no longer have the characteristics of single-sex. It's a doubt that if students from Physical Education Department have masculine tendencies as general stereotypes. The purpose of the study was to examine cognition and differentiation of sex role of those students who majored in Physical Education Department in National Taiwan Normal University and offered a foundation for exploring sex role and self -concept of physical education teachers in the future.The subjects were 83 males and 59 females (N=162) in sophomore, junior and senior year of the Physical Education Department. The study used a questionnaire survey. Data was collected via Lee's Sex Role Inventory and Tennessee Self Concept Scale, and analyzed by one-way ANOVA.Results revealed that students of Physical Education Department with different grades and genders were no significant difference in the sex role differentiation, students of Physical Education Department with different gender and self-concept are significantly different, students of Physical Education Department with different grades have no significant difference in self -concept, students of Physical Education Department have significant difference in sex role differentiation and self -concept, and students of Physical Education Department have a high proportion of the population with androgyny in sex roles.It concluded that the study did not include the course content, learning environment, family environment, and other factors. Future research can be added and do in-depth study. This current study suggested that it can let students experience the masculine and feminine traits of the curriculum content in the future course. It can also help the students adapt to the modern environment.The study concluded that future research can add course content, learning environment, home environment and other factors to do further examine. It suggested that teachers can make students experience the course content with both characteristics of masculinity and femininity in the future courses so that students could be much easier to adapt to the environment.