The purpose of this research was to design and conduct a performance assessment on students' ability on implementing experiments. Its effects were also discussed. Related ability indicators were transformed into assessment objectives. There were four essential elements in the assessment objectives: performance task, response format, scoring rubrics, and meta rubrics, to ensure the quality of assessment design. After the pilot test with 26 students and formal test with 25 students, a two-stage assessing procedure was applied to motivate students' learning and understanding. Pearson's r, Cronbach's α, and Kappa's κ all indicated the consistent rating results from two raters. This study concluded that the two-stage four-element performance assessment functioned as a mediator to depict students' ability structure, enhance their designing ability and provide teachers with feedback on teaching.