This study is aimed at exploring the application of E-Learning in the design of calculus tutorial website. The major purpose of this study was to explore how to integrate E-Learning into Calculus teaching in university and to assess its effectiveness upon the Calculus learning of the National Formosa Universit students. The quasi-experimental design was used to compare the effect of the E-Learning and traditional method. The 97 university students, who take in the Calculus course, were invited to participate in the study. In this research, A class of 51 students were picked up randomly as the experimental group, while the other class was the control group. After a semester, data were collected in the study. The result revealed that: there are no distinguished differences in parents' education background and family economy. There were significant differences on "Learning Value" and "Relations regarding the Interaction between Teacher-Student and among the Peer Group of Students". There was no significant difference on Improvement Of Learning Achievements between the experimental group and the control group. There was no significant difference on Self-efficacy between the experimental group and the control group. There was significant difference on the achievement post-test between the experimental group and the control group.