This study was to construct learning organizational culture scale by means of the theories of organizational culture for middle school physical education sector. Confirmatory factor analysis (CFA) was used to examine the proposed model to establish the reliability and validity of the scale. 192 valid questionnaires were collected. The model of learning organizational culture included six factors: knowledge sharing and communication, management support, deep dialogue, knowledge community, shared value, cooperation. The results showed that the model had an acceptable fitting and also had convergent validity, discriminant validity, and good reliability.