Today, the presentation of online mathematical instructional materials tends to be sequential and one-way. As a result, students obtain fragmental and vague concepts after browsing Web pages containing these materials. In addition, when students want to evaluate their learning performance, what they can receive is only a grade, instead of the specific explanations about their mistakes or misconceptions. To address the above problems, this work aims to build an on-line assessment and misconception correction tool for those who learn fundamental mathematics. The students learn how to solve polynomial equations through the tool. Whenever students make any recognized mistakes during the solving process, the tool instantly assists them to explore the reason of making mistake. These responsive feedbacks are helpful to guide them toward the correct direction during their cognitive processes. Thus, this tool can be used as a teaching assistant for remedial learning. To evaluate the effectiveness of this tool, a quasi-experimental design was implemented in this study. This research sampled two classes (Grade) that enrolled in Tachia Junior High School in Taichung County. One is taken as the experimental group and the other as the control group. During a five-week period, the experimental group accepted total ten-hour remedial learning through the tool, while the control group takes traditional instruction. After every week of remedial learning, a post-test is conducted in order to collect data for statistical analysis. The collected data is analyzed by T-test, One-way ANCOVA and descriptive statistic method. The findings through this work are described as follows: 1. On-line assessment and misconception correction tool can diagnose the different mistakes of students. It could serve as an assistant for adaptive remedial instruction. 2. After five weeks of trial, the students who utilized the tool obtained significantly higher learning achievement than those who enrolled in the traditional instructional environment. It implies that correcting students' misconception about Mathematics should not be regarded as a short-term task. This phenomenon further reveals the value of this tool in reducing instructors' burden with repetitive and long-term correction tasks.